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36.Microwave Theory and Techniques by John G. Webster (Editor)

By John G. Webster (Editor)

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Nucleic Acids Res 18 : 4253โ€“ 4254 38. Paik SH, Chakicherla A, Hansen JN (1998) Identification and Characterization of the Structural and Transporter Genes for, and the Chemical and Biological Properties of Sublancin 168, a Novel lantibiotic Produced by Bacillus subtilis 168. J Biol Chem 273 : 23134โ€“23142 39. Jung G (1991) Lantibiotics: a survey. In: Jung G, Sahl H-G (eds) Nisin and Novel Lantibiotics. ESCOM Science, Leiden, pp 1โ€“34 52 E. Sablon et al. 40. De Vos WM, Kuipers OP, van der Meer JR, Siezen RJ (1995b) Maturation pathway of nisin and other lantibiotics: post-translational modified antimicrobial peptides exported by Gram-positive bacteria.

Therefore, the leader peptide might have an essential role in biosynthesis, either in that it could contain a specific recognition motif which would direct the precursor towards biosynthetic enzymes and/or in that the leader peptide may interact with the propeptide region to stabilize a conformation which is essential for correct modification [39]. In contrast, the class IAII lantibiotics contain a double-glycine type processing site and a leader peptide that matches the consensus of the non-lantibiotic class II bacteriocins [21, 40] (Fig.

Besides PlnP, PlnM could hence be considered as a second valid candidate for PlnN immunity, although located in the 5ยข end of the operon. Two proteins, PlnG (ABC transporter) and plnH (accessory protein), shown to constitute an ATP-dependent transport apparatus, were located downstream of the plnABCD operon [49]. The homologous counterparts in the sakacin A operon were named SapT and SapE, respectively [133]. The region encoding plantaricin A activity has proven to be a multiple gene locus consisting of not less than 22 different open reading frames in the same or opposite orientation to the previously described plnABCD operon [49, 134].

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